Carbon Nanofiber Arrays: A Novel Tool for Microdelivery of Biomolecules to Plants.
Identifieur interne : 001982 ( Main/Exploration ); précédent : 001981; suivant : 001983Carbon Nanofiber Arrays: A Novel Tool for Microdelivery of Biomolecules to Plants.
Auteurs : Sandra M. Davern [États-Unis] ; Timothy E. Mcknight [États-Unis] ; Robert F. Standaert [États-Unis] ; Jennifer L. Morrell-Falvey [États-Unis] ; Elena D. Shpak [États-Unis] ; Udaya C. Kalluri [États-Unis] ; Joanna Jelenska [États-Unis] ; Jean T. Greenberg [États-Unis] ; Saed Mirzadeh [États-Unis]Source :
- PloS one [ 1932-6203 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Carbone, Nanofibres.
- métabolisme : Arbres, Feuilles de plante, Populus.
- méthodes : Techniques de biocapteur.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Carbon.
- chemistry : Nanofibers.
- metabolism : Plant Leaves, Populus, Trees.
- methods : Biosensing Techniques.
Abstract
Effective methods for delivering bioprobes into the cells of intact plants are essential for investigating diverse biological processes. Increasing research on trees, such as Populus spp., for bioenergy applications is driving the need for techniques that work well with tree species. This report introduces vertically aligned carbon nanofiber (VACNF) arrays as a new tool for microdelivery of labeled molecules to Populus leaf tissue and whole plants. We demonstrated that VACNFs penetrate the leaf surface to deliver sub-microliter quantities of solution containing fluorescent or radiolabeled molecules into Populus leaf cells. Importantly, VACNFs proved to be gentler than abrasion with carborundum, a common way to introduce material into leaves. Unlike carborundum, VACNFs did not disrupt cell or tissue integrity, nor did they induce production of hydrogen peroxide, a typical wound response. We show that femtomole to picomole quantities of labeled molecules (fluorescent dyes, small proteins and dextran), ranging from 0.5-500 kDa, can be introduced by VACNFs, and we demonstrate the use of the approach to track delivered probes from their site of introduction on the leaf to distal plant regions. VACNF arrays thus offer an attractive microdelivery method for the introduction of biomolecules and other probes into trees and potentially other types of plants.
DOI: 10.1371/journal.pone.0153621
PubMed: 27119338
PubMed Central: PMC4847769
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Carbon Nanofiber Arrays: A Novel Tool for Microdelivery of Biomolecules to Plants.</title><author><name sortKey="Davern, Sandra M" sort="Davern, Sandra M" uniqKey="Davern S" first="Sandra M" last="Davern">Sandra M. Davern</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Mcknight, Timothy E" sort="Mcknight, Timothy E" uniqKey="Mcknight T" first="Timothy E" last="Mcknight">Timothy E. Mcknight</name><affiliation wicri:level="2"><nlm:affiliation>Electrical & Electronics Systems Research Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Electrical & Electronics Systems Research Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Standaert, Robert F" sort="Standaert, Robert F" uniqKey="Standaert R" first="Robert F" last="Standaert">Robert F. Standaert</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Biology & Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biology & Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Morrell Falvey, Jennifer L" sort="Morrell Falvey, Jennifer L" uniqKey="Morrell Falvey J" first="Jennifer L" last="Morrell-Falvey">Jennifer L. Morrell-Falvey</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Shpak, Elena D" sort="Shpak, Elena D" uniqKey="Shpak E" first="Elena D" last="Shpak">Elena D. Shpak</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Kalluri, Udaya C" sort="Kalluri, Udaya C" uniqKey="Kalluri U" first="Udaya C" last="Kalluri">Udaya C. Kalluri</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Jelenska, Joanna" sort="Jelenska, Joanna" uniqKey="Jelenska J" first="Joanna" last="Jelenska">Joanna Jelenska</name><affiliation wicri:level="4"><nlm:affiliation>Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region><settlement type="city">Chicago</settlement></placeName><orgName type="university">Université de Chicago</orgName></affiliation></author><author><name sortKey="Greenberg, Jean T" sort="Greenberg, Jean T" uniqKey="Greenberg J" first="Jean T" last="Greenberg">Jean T. Greenberg</name><affiliation wicri:level="4"><nlm:affiliation>Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region><settlement type="city">Chicago</settlement></placeName><orgName type="university">Université de Chicago</orgName></affiliation></author><author><name sortKey="Mirzadeh, Saed" sort="Mirzadeh, Saed" uniqKey="Mirzadeh S" first="Saed" last="Mirzadeh">Saed Mirzadeh</name><affiliation wicri:level="2"><nlm:affiliation>Nuclear Security & Isotope Technology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Nuclear Security & Isotope Technology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27119338</idno><idno type="pmid">27119338</idno><idno type="doi">10.1371/journal.pone.0153621</idno><idno type="pmc">PMC4847769</idno><idno type="wicri:Area/Main/Corpus">001815</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001815</idno><idno type="wicri:Area/Main/Curation">001815</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001815</idno><idno type="wicri:Area/Main/Exploration">001815</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Carbon Nanofiber Arrays: A Novel Tool for Microdelivery of Biomolecules to Plants.</title><author><name sortKey="Davern, Sandra M" sort="Davern, Sandra M" uniqKey="Davern S" first="Sandra M" last="Davern">Sandra M. Davern</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Mcknight, Timothy E" sort="Mcknight, Timothy E" uniqKey="Mcknight T" first="Timothy E" last="Mcknight">Timothy E. Mcknight</name><affiliation wicri:level="2"><nlm:affiliation>Electrical & Electronics Systems Research Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Electrical & Electronics Systems Research Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Standaert, Robert F" sort="Standaert, Robert F" uniqKey="Standaert R" first="Robert F" last="Standaert">Robert F. Standaert</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Biology & Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biology & Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Morrell Falvey, Jennifer L" sort="Morrell Falvey, Jennifer L" uniqKey="Morrell Falvey J" first="Jennifer L" last="Morrell-Falvey">Jennifer L. Morrell-Falvey</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Shpak, Elena D" sort="Shpak, Elena D" uniqKey="Shpak E" first="Elena D" last="Shpak">Elena D. Shpak</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Kalluri, Udaya C" sort="Kalluri, Udaya C" uniqKey="Kalluri U" first="Udaya C" last="Kalluri">Udaya C. Kalluri</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Jelenska, Joanna" sort="Jelenska, Joanna" uniqKey="Jelenska J" first="Joanna" last="Jelenska">Joanna Jelenska</name><affiliation wicri:level="4"><nlm:affiliation>Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region><settlement type="city">Chicago</settlement></placeName><orgName type="university">Université de Chicago</orgName></affiliation></author><author><name sortKey="Greenberg, Jean T" sort="Greenberg, Jean T" uniqKey="Greenberg J" first="Jean T" last="Greenberg">Jean T. Greenberg</name><affiliation wicri:level="4"><nlm:affiliation>Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region><settlement type="city">Chicago</settlement></placeName><orgName type="university">Université de Chicago</orgName></affiliation></author><author><name sortKey="Mirzadeh, Saed" sort="Mirzadeh, Saed" uniqKey="Mirzadeh S" first="Saed" last="Mirzadeh">Saed Mirzadeh</name><affiliation wicri:level="2"><nlm:affiliation>Nuclear Security & Isotope Technology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Nuclear Security & Isotope Technology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (methods)</term><term>Carbon (chemistry)</term><term>Nanofibers (chemistry)</term><term>Plant Leaves (metabolism)</term><term>Populus (metabolism)</term><term>Trees (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (métabolisme)</term><term>Carbone (composition chimique)</term><term>Feuilles de plante (métabolisme)</term><term>Nanofibres (composition chimique)</term><term>Populus (métabolisme)</term><term>Techniques de biocapteur (méthodes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Carbon</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nanofibers</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Carbone</term><term>Nanofibres</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arbres</term><term>Feuilles de plante</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Techniques de biocapteur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Effective methods for delivering bioprobes into the cells of intact plants are essential for investigating diverse biological processes. Increasing research on trees, such as Populus spp., for bioenergy applications is driving the need for techniques that work well with tree species. This report introduces vertically aligned carbon nanofiber (VACNF) arrays as a new tool for microdelivery of labeled molecules to Populus leaf tissue and whole plants. We demonstrated that VACNFs penetrate the leaf surface to deliver sub-microliter quantities of solution containing fluorescent or radiolabeled molecules into Populus leaf cells. Importantly, VACNFs proved to be gentler than abrasion with carborundum, a common way to introduce material into leaves. Unlike carborundum, VACNFs did not disrupt cell or tissue integrity, nor did they induce production of hydrogen peroxide, a typical wound response. We show that femtomole to picomole quantities of labeled molecules (fluorescent dyes, small proteins and dextran), ranging from 0.5-500 kDa, can be introduced by VACNFs, and we demonstrate the use of the approach to track delivered probes from their site of introduction on the leaf to distal plant regions. VACNF arrays thus offer an attractive microdelivery method for the introduction of biomolecules and other probes into trees and potentially other types of plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27119338</PMID><DateCompleted><Year>2017</Year><Month>02</Month><Day>17</Day></DateCompleted><DateRevised><Year>2019</Year><Month>02</Month><Day>19</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><Issue>4</Issue><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Carbon Nanofiber Arrays: A Novel Tool for Microdelivery of Biomolecules to Plants.</ArticleTitle><Pagination><MedlinePgn>e0153621</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0153621</ELocationID><Abstract><AbstractText>Effective methods for delivering bioprobes into the cells of intact plants are essential for investigating diverse biological processes. Increasing research on trees, such as Populus spp., for bioenergy applications is driving the need for techniques that work well with tree species. This report introduces vertically aligned carbon nanofiber (VACNF) arrays as a new tool for microdelivery of labeled molecules to Populus leaf tissue and whole plants. We demonstrated that VACNFs penetrate the leaf surface to deliver sub-microliter quantities of solution containing fluorescent or radiolabeled molecules into Populus leaf cells. Importantly, VACNFs proved to be gentler than abrasion with carborundum, a common way to introduce material into leaves. Unlike carborundum, VACNFs did not disrupt cell or tissue integrity, nor did they induce production of hydrogen peroxide, a typical wound response. We show that femtomole to picomole quantities of labeled molecules (fluorescent dyes, small proteins and dextran), ranging from 0.5-500 kDa, can be introduced by VACNFs, and we demonstrate the use of the approach to track delivered probes from their site of introduction on the leaf to distal plant regions. VACNF arrays thus offer an attractive microdelivery method for the introduction of biomolecules and other probes into trees and potentially other types of plants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Davern</LastName><ForeName>Sandra M</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McKnight</LastName><ForeName>Timothy E</ForeName><Initials>TE</Initials><AffiliationInfo><Affiliation>Electrical & Electronics Systems Research Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Standaert</LastName><ForeName>Robert F</ForeName><Initials>RF</Initials><Identifier Source="ORCID">0000-0002-5684-1322</Identifier><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biology & Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morrell-Falvey</LastName><ForeName>Jennifer L</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shpak</LastName><ForeName>Elena D</ForeName><Initials>ED</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Cellular & Molecular Biology, University of Tennessee, Knoxville, Tennessee, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kalluri</LastName><ForeName>Udaya C</ForeName><Initials>UC</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jelenska</LastName><ForeName>Joanna</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Greenberg</LastName><ForeName>Jean T</ForeName><Initials>JT</Initials><AffiliationInfo><Affiliation>Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mirzadeh</LastName><ForeName>Saed</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Nuclear Security & Isotope Technology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>04</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>7440-44-0</RegistryNumber><NameOfSubstance UI="D002244">Carbon</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015374" MajorTopicYN="N">Biosensing Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002244" MajorTopicYN="N">Carbon</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057139" MajorTopicYN="N">Nanofibers</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>02</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>03</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>4</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>4</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>2</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27119338</ArticleId><ArticleId IdType="doi">10.1371/journal.pone.0153621</ArticleId><ArticleId IdType="pii">PONE-D-16-07656</ArticleId><ArticleId IdType="pmc">PMC4847769</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Int Arch Allergy Appl Immunol. 1966;29(2):185-9</Citation><ArticleIdList><ArticleId IdType="pubmed">4160044</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1983 Mar;157(2):124-30</Citation><ArticleIdList><ArticleId IdType="pubmed">24264065</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1987 Apr;83(4):941-4</Citation><ArticleIdList><ArticleId IdType="pubmed">16665368</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2009;513:131-52</Citation><ArticleIdList><ArticleId IdType="pubmed">19347644</ArticleId></ArticleIdList></Reference><Reference><Citation>Ecol Lett. 2007 Jun;10(6):490-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17498148</ArticleId></ArticleIdList></Reference><Reference><Citation>Biophys J. 2004 Dec;87(6):4259-70</Citation><ArticleIdList><ArticleId IdType="pubmed">15361408</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1999 Jan;119(1):17-20</Citation><ArticleIdList><ArticleId IdType="pubmed">9880341</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2014 Sep;166(1):306-13</Citation><ArticleIdList><ArticleId IdType="pubmed">25056922</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1983 Aug;158(4):320-8</Citation><ArticleIdList><ArticleId IdType="pubmed">24264752</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1990 Jul;181(4):555-9</Citation><ArticleIdList><ArticleId IdType="pubmed">24196937</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1986 Oct;82(2):432-42</Citation><ArticleIdList><ArticleId IdType="pubmed">16665047</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Bot. 1965 Feb;52:120-6</Citation><ArticleIdList><ArticleId IdType="pubmed">14268296</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):14150-5</Citation><ArticleIdList><ArticleId IdType="pubmed">9391168</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1985 Jul;164(4):473-9</Citation><ArticleIdList><ArticleId IdType="pubmed">24248219</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1988 Nov;88(3):667-70</Citation><ArticleIdList><ArticleId IdType="pubmed">16666366</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Biol. 2011 Jun 7;21(11):927-32</Citation><ArticleIdList><ArticleId IdType="pubmed">21620705</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):2227-31</Citation><ArticleIdList><ArticleId IdType="pubmed">15668382</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2010 Jun;61(6):1711-7</Citation><ArticleIdList><ArticleId IdType="pubmed">20181661</ArticleId></ArticleIdList></Reference><Reference><Citation>Lab Chip. 2011 Oct 21;11(20):3523-9</Citation><ArticleIdList><ArticleId IdType="pubmed">21879140</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2006;57(4):729-37</Citation><ArticleIdList><ArticleId IdType="pubmed">16473888</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1999 Dec 14;38(50):16424-31</Citation><ArticleIdList><ArticleId IdType="pubmed">10600103</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2008 Mar;53(5):739-49</Citation><ArticleIdList><ArticleId IdType="pubmed">18005229</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2011 Jan;6(1):36-45</Citation><ArticleIdList><ArticleId IdType="pubmed">21212781</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1989 Oct 20;246(4928):377-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16552920</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1974 Dec;54(6):892-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16658995</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1987 Jun;171(2):145-57</Citation><ArticleIdList><ArticleId IdType="pubmed">24227322</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1979 Jun;63(6):1072-5</Citation><ArticleIdList><ArticleId IdType="pubmed">16660859</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 1986 Dec;2(1_2_3):289-299</Citation><ArticleIdList><ArticleId IdType="pubmed">14975862</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Sep 15;313(5793):1596-604</Citation><ArticleIdList><ArticleId IdType="pubmed">16973872</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11945-50</Citation><ArticleIdList><ArticleId IdType="pubmed">16087887</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4524-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18334638</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1999 Jan 1;283(5398):94-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9872750</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2005;303:191-208</Citation><ArticleIdList><ArticleId IdType="pubmed">15923685</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol Methods. 2007 Jun;142(1-2):198-203</Citation><ArticleIdList><ArticleId IdType="pubmed">17337069</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2000 Jan;41(1):124-8</Citation><ArticleIdList><ArticleId IdType="pubmed">10750718</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2011 Apr;21(4):634-41</Citation><ArticleIdList><ArticleId IdType="pubmed">21367939</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2009;513:111-30</Citation><ArticleIdList><ArticleId IdType="pubmed">19347645</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 1996 Dec;16(3-4):127-32</Citation><ArticleIdList><ArticleId IdType="pubmed">24177537</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1989 Aug;179(1):24-31</Citation><ArticleIdList><ArticleId IdType="pubmed">24201418</ArticleId></ArticleIdList></Reference><Reference><Citation>ACS Nano. 2008 Jan;2(1):69-76</Citation><ArticleIdList><ArticleId IdType="pubmed">19206549</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2012 Feb;69(4):713-9</Citation><ArticleIdList><ArticleId IdType="pubmed">22004025</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1994 Jan;104(1):17-27</Citation><ArticleIdList><ArticleId IdType="pubmed">12232057</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1988 Dec;176(4):533-40</Citation><ArticleIdList><ArticleId IdType="pubmed">24220950</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Plant Sci. 2016 Jan 11;4(1):null</Citation><ArticleIdList><ArticleId IdType="pubmed">26819863</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Biol. 2011 Jul 26;21(14):1197-203</Citation><ArticleIdList><ArticleId IdType="pubmed">21757352</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2013;1069:215-26</Citation><ArticleIdList><ArticleId IdType="pubmed">23996318</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1999 May 25;96(11):6553-7</Citation><ArticleIdList><ArticleId IdType="pubmed">10339626</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2000 May;123(1):125-38</Citation><ArticleIdList><ArticleId IdType="pubmed">10806231</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Methods. 2012 Aug 07;8(1):30</Citation><ArticleIdList><ArticleId IdType="pubmed">22871142</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1433-7</Citation><ArticleIdList><ArticleId IdType="pubmed">8108427</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2005 Jun;17(6):1801-14</Citation><ArticleIdList><ArticleId IdType="pubmed">15863519</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Illinois</li><li>Tennessee</li></region><settlement><li>Chicago</li></settlement><orgName><li>Université de Chicago</li></orgName></list><tree><country name="États-Unis"><region name="Tennessee"><name sortKey="Davern, Sandra M" sort="Davern, Sandra M" uniqKey="Davern S" first="Sandra M" last="Davern">Sandra M. Davern</name></region><name sortKey="Greenberg, Jean T" sort="Greenberg, Jean T" uniqKey="Greenberg J" first="Jean T" last="Greenberg">Jean T. Greenberg</name><name sortKey="Jelenska, Joanna" sort="Jelenska, Joanna" uniqKey="Jelenska J" first="Joanna" last="Jelenska">Joanna Jelenska</name><name sortKey="Kalluri, Udaya C" sort="Kalluri, Udaya C" uniqKey="Kalluri U" first="Udaya C" last="Kalluri">Udaya C. Kalluri</name><name sortKey="Mcknight, Timothy E" sort="Mcknight, Timothy E" uniqKey="Mcknight T" first="Timothy E" last="Mcknight">Timothy E. Mcknight</name><name sortKey="Mirzadeh, Saed" sort="Mirzadeh, Saed" uniqKey="Mirzadeh S" first="Saed" last="Mirzadeh">Saed Mirzadeh</name><name sortKey="Morrell Falvey, Jennifer L" sort="Morrell Falvey, Jennifer L" uniqKey="Morrell Falvey J" first="Jennifer L" last="Morrell-Falvey">Jennifer L. Morrell-Falvey</name><name sortKey="Morrell Falvey, Jennifer L" sort="Morrell Falvey, Jennifer L" uniqKey="Morrell Falvey J" first="Jennifer L" last="Morrell-Falvey">Jennifer L. Morrell-Falvey</name><name sortKey="Shpak, Elena D" sort="Shpak, Elena D" uniqKey="Shpak E" first="Elena D" last="Shpak">Elena D. Shpak</name><name sortKey="Standaert, Robert F" sort="Standaert, Robert F" uniqKey="Standaert R" first="Robert F" last="Standaert">Robert F. Standaert</name><name sortKey="Standaert, Robert F" sort="Standaert, Robert F" uniqKey="Standaert R" first="Robert F" last="Standaert">Robert F. Standaert</name><name sortKey="Standaert, Robert F" sort="Standaert, Robert F" uniqKey="Standaert R" first="Robert F" last="Standaert">Robert F. Standaert</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001982 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001982 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:27119338
|texte= Carbon Nanofiber Arrays: A Novel Tool for Microdelivery of Biomolecules to Plants.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:27119338" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |